Cancer Immunosurveillance - Methods and Protocols

This volume explores the latest techniques used to study tumor immunology. The chapters in this book detail methodologies for functional analysis and expansion of T lymphocytes for cancer research. The chapters also cover topics such as how single-cell RNA-sequencing can be exploited to dissect immune cell heterogeneity and precursors; isolating and expanding natural killer (NK) cells; evaluating NK cell-mediated anti-tumor killing activity in vitro; immunosurveillance orchestrated by specific immune subsets; use of HLA peptidomics for cancer-exome based identification of tumor neo-antigens; gold standard assessment of immunogenic cell death in oncological mouse models and methods to look at the therapeutic relevance of immune modulation in cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory pro tocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Cancer Immunosurveillance: Methods and Protocols is a valuable resource to aid researchers in better understanding and experimenting in this exciting and developing field.

Inhaltsverzeichnis:

Usage of Droplet Digital PCR (ddPCR) Assays for T Cell Quantification in Cancer.- An Economical, Quantitative, and Robust Protocol for High Throughput T Cell Receptor Sequencing from Tumor or Blood.- Generation of Tumor Antigen-Specific Cytotoxic T Lymphocytes from Pluripotent Stem Cells.- Expansion and Adoptive Transfer of Human Vd2+ T Cells to Assess Anti-Tumor Effects In Vivo.- Usage of Single-Cell RNA-Sequencing to Unveil Immune Lymphoid Cell Precursors.- Isolation, Expansion, and Characterization of Natural Killer Cells and Their Precursors as a Tool to Study Cancer Immunosurveillance.- CD107a Degranulation Assay to Evaluate Immune Cell Anti-Tumor Activity.- A Flow Cytometric NK Cell-Mediated Cytotoxicity Assay to Evaluate Anti-Cancer Immune Responses In Vitro.- Mouse Models to Study Natural Killer Cell-Mediated Immunosurveillance and Metastatic Latency.- Tumor-Associated Macrophage Isolation and In Vivo Analysis of their Tumor Promoting Activity.- Conditional Genetic Ablatio n Mouse Models as a Tool to Study Cancer Immunosurveillance In Vivo.- Determination and Isolation of Immune Populations from Brain Tumor Microenvironments.- Mouse Model of Colitis Associated Colorectal Cancer (CAC): Isolation and Characterization of Mucosal-Associated Lymphoid Cells.- Cancer Exome-Based Identification of Tumor Neo-Antigens Using Mass Spectrometry.- Single-Cell Mass Cytometry of Archived Human Epithelial Tissue for Decoding Cancer Signaling Pathways.- Purification of Leukemia-Derived Exosomes to Study Microenvironment Modulation.- Molecular Characterization of Circulating Tumor Cells to Study Cancer Immunoevasion.- Quantitative Identification of Senescent Cells in Cancer.- Generation and Harnessing of Heterotypic Tumor-Stroma Spheroids to Study Cancer Immunosurveillance.- Establishment of Slice Cultures as a Tool to Study the Cancer Immune Microenvironment.- Gold StandardAssessment of Immunogenic Cell Death in Oncological Mouse Models.- A Novel Dendritic Cell-Based Vaccination Protocol to Stimulate Immunosurveillance of Aggressive Cancers.- Development of Bi-Specific Antibody Derivatives for Cancer Immunotherapy.- Generation of CAR-T Cells for Cancer Immunotherapy.